PT Tawada Healthcare Semarang Branch is one of PT Tawada Healthcare office branch, a company engaged in medical devices provider that has been established since 1999. PT Tawada Healthcare has 13 branches throughout Indonesia and also provides medical devices in many hospitals in Indonesia. This research aims to determine the effect of motivation, working environment, and career development toward employees’ loyalty at the PT Tawada Healthcare Semarang, which is one of the regional offices of PT Tawada Healthcare. The data collection method used in this research are questionnaires, literature study, and interview which were distributed to 35 respondents using Total Sampling. The methods of data analysis used are validity test, reliability test, classical assumption test, T test, F test, and coefficient of determination. The results of this research shows that motivation, working environment, and career development have positive and significant influence either partially or simultaneously on employees’ loyalty. The result of the analysis obtained Adjusted R Square value is 0.813, it means that the contribution of the independent variable in influencing the dependent variable is 81.3%, while the remaining 0.197 or 19.7% is influenced by other factors which are not examined in this research