PT. JasaMarga (Persero) Tbk is one of the company which manages highways inIndonesia. The purpose of this research is to analyze the influence of service quality that is tangible (X1), reliability (X2), and responsiveness (X3) to satisfaction highway users at PT JasaMarga (Persero) Tbk Semarang Branch. The methods of data collection used were interviews, questionnaires and literature studies. This study applied incidental sampling with a sample of 50 respondents who were highway users at PT JasaMarga (Persero) TbkSemarang Branch. The methods of analyzing the data were validity, reliability, multiple linear regressions, t-test, and f-test. The analysis was processed by SPSS 21.0 version. Based on the results of multiple linear regression analysis it was found that the regression equation Y = 0,399 + 0,284X1 + 0,258X2 + 0,329X3. In addition, test results F is 55.727, a mean value of 0.000 sign value ≤ alpha (α) 0,05. So it could be concluded the quality of service which consist of tangibility (X1), reliability (X2), and responsiveness (X3) had a possitive and significance influence to satisfaction of highway users at PT JasaMarga (Persero) Tbk Semarang Branch.